Abstract
A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Fluorescence
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HeLa Cells
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Humans
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Inteins / physiology*
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Light*
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Maltose-Binding Proteins / metabolism*
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Protein Engineering
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Protein Splicing / physiology*
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Recombinant Proteins / chemistry*
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Recombinant Proteins / metabolism*
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Recombinant Proteins / radiation effects
Substances
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Maltose-Binding Proteins
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Recombinant Proteins