Abstract
microRNAs play an important role in the progression of hepatocellular carcinoma (HCC). In this study, we found that miR-582-5p expression was downregulated in hepatoma tissues and HCC cell lines. Upregulation of miR-582-5p reduced colony number, inhibited cellular proliferation, and arrested cell cycle in G0/G1 phase. When miR-582-5p was inhibited, the colony number was increased and cellular proliferation and cell cycle were promoted. Further studies showed that miR-582-5p regulated the progression of HCC through directly inhibiting the expression of CDK1 and AKT3, and indirectly inhibiting the expression of cyclinD1.
Keywords:
AKT3; CDK1; CyclinD1; Hepatocellular carcinoma; miR-582-5p.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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CDC2 Protein Kinase
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Carcinoma, Hepatocellular / genetics*
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Carcinoma, Hepatocellular / pathology
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Cell Cycle / genetics
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Cell Line, Tumor
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Cell Proliferation / genetics
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Cyclin-Dependent Kinases / biosynthesis*
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Cyclin-Dependent Kinases / genetics
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Gene Expression Regulation, Neoplastic
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Humans
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Liver Neoplasms / genetics*
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Liver Neoplasms / pathology
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MicroRNAs / antagonists & inhibitors
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MicroRNAs / genetics*
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Proto-Oncogene Proteins c-akt / biosynthesis*
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Proto-Oncogene Proteins c-akt / genetics
Substances
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MIRN582 microRNA, human
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MicroRNAs
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AKT3 protein, human
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Proto-Oncogene Proteins c-akt
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CDC2 Protein Kinase
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CDK1 protein, human
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Cyclin-Dependent Kinases