Abstract
The transcription factor MYC is a proto-oncogene regulating cell proliferation, cell cycle, apoptosis and metabolism. The recent identification of MYC-regulated long noncoding RNAs (lncRNAs) expands our knowledge of the role of lncRNAs in MYC functions. Here, we identify MYC-repressed lncRNAs named MYCLo-4, -5 and -6 by comparing 3 categories of lncRNAs (downregulated in highly MYC-expressing colorectal cancer, up-regulated by MYC knockdown in HCT116, upregulated by MYC knockdown in RKO). The MYC-repressed MYCLos are implicated in MYC-modulated cell proliferation through cell cycle regulation. By screening cell cycle-related genes regulated by MYC and the MYC-repressed MYCLos, we identified the MYC-repressed gene GADD45A as a target gene of the MYC-repressed MYCLos such as MYCLo-4 and MYCLo-6.
Keywords:
MYC; cell cycle; cell proliferation; long noncoding RNA.
Publication types
-
Research Support, N.I.H., Extramural
MeSH terms
-
Amino Acid Sequence
-
Cell Cycle / genetics
-
Cell Cycle Proteins / genetics
-
Cell Proliferation / genetics
-
Disease Progression
-
Gene Knockdown Techniques
-
Genes, myc
-
HCT116 Cells
-
Humans
-
Molecular Sequence Data
-
Nuclear Proteins / genetics
-
Promoter Regions, Genetic
-
Proto-Oncogene Mas
-
Proto-Oncogene Proteins c-myc / genetics*
-
RNA, Long Noncoding / genetics*
-
RNA, Small Interfering / administration & dosage
-
RNA, Small Interfering / genetics
-
Transfection
Substances
-
Cell Cycle Proteins
-
GADD45A protein, human
-
MAS1 protein, human
-
MYC protein, human
-
Nuclear Proteins
-
Proto-Oncogene Mas
-
Proto-Oncogene Proteins c-myc
-
RNA, Long Noncoding
-
RNA, Small Interfering
Associated data
-
GENBANK/JX046912
-
GENBANK/JX046913
-
GENBANK/JX046914
-
GENBANK/JX046915
-
GEO/GSE39077
-
GEO/GSM953006
-
GEO/GSM953007
-
GEO/GSM953008
-
GEO/GSM955335
-
GEO/GSM955336
-
GEO/GSM955337
-
GEO/GSM955338
-
GEO/GSM955339
-
GEO/GSM955340
-
GEO/GSM955341
-
GEO/GSM955342
-
GEO/GSM955343
-
GEO/GSM955344
-
GEO/GSM955345
-
GEO/GSM955346