Deep Sequencing of HIV-1 in Cerebrospinal Fluid

Clin Infect Dis. 2015 Sep 15;61(6):1022-5. doi: 10.1093/cid/civ417. Epub 2015 May 28.

Abstract

Using deep sequencing, human immunodeficiency virus (HIV) resistance-associated mutations were detected as minority species in the cerebrospinal fluid (CSF) of 4 patients with higher HIV type 1 RNA load in CSF than in plasma, but not in 2 patients with higher plasma viral load. Deep sequencing could help our understanding of viral escape in the central nervous system.

Keywords: CNS penetration; deep sequencing; minority species; viral escape.

MeSH terms

  • Adult
  • Cerebrospinal Fluid / virology*
  • Drug Resistance, Viral
  • Female
  • Genetic Variation*
  • HIV Infections / virology*
  • HIV-1 / classification*
  • HIV-1 / drug effects
  • HIV-1 / genetics*
  • HIV-1 / isolation & purification
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Male
  • Middle Aged
  • Mutation
  • Retrospective Studies
  • Viral Load