Characterization of α-, β- and p120-Catenin Expression in Feline Mammary Tissues and their Relation with E- and P-Cadherin

Anticancer Res. 2015 Jun;35(6):3361-9.

Abstract

Background: Abnormal catenin expression has been related to mammary carcinogenesis in both human and canine species and they are considered tumor- and invasion-suppressor molecules; however, in feline mammary tissues they have been scarcely studied.

Materials and methods: The immunohistochemical expression of α-, β- and p120-catenin was studied in a series of normal feline mammary glands, hyperplastic/dysplastic lesions and benign and malignant mammary tumors. Their relationship with clinicopathological parameters and with E- and P-cadherin expression was assessed.

Results: Normal tissues, hyperplastic/dysplastic lesions and benign tumors expressed α-, β- and p120-catenin in the membrane of more than 75% of the luminal epithelial cells, while in malignant tumors, there was a reduction in their membranous expression and a p120-catenin cytoplasmic expression in 40%. Reduced α-catenin expression was related to tumor features with prognostic value, namely tumor size (p=0.0203) and necrosis (p=0.0205). The expression of α-, β- and p120-catenin were individually related to each other and collectively associated with E-cadherin expression.

Conclusion: The results demonstrate a relationship between feline mammary carcinogenesis and decreased expression of catenins, suggesting that they may represent a valuable tool in the diagnosis of feline mammary neoplasms.

Keywords: Feline; cadherins; catenins; mammary tumours.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers, Tumor / biosynthesis
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • Breast Neoplasms / veterinary
  • Cadherins / biosynthesis*
  • Carcinogenesis / genetics
  • Catenins / biosynthesis*
  • Cats
  • Delta Catenin
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • Mammary Glands, Animal / metabolism
  • Mammary Glands, Animal / pathology
  • alpha Catenin / biosynthesis*
  • beta Catenin / biosynthesis*

Substances

  • Biomarkers, Tumor
  • Cadherins
  • Catenins
  • alpha Catenin
  • beta Catenin
  • Delta Catenin