Genotypic Variation in the Concentration of β-N-Oxalyl-L-α,β-diaminopropionic Acid (β-ODAP) in Grass Pea (Lathyrus sativus L.) Seeds Is Associated with an Accumulation of Leaf and Pod β-ODAP during Vegetative and Reproductive Stages at Three Levels of Water Stress

J Agric Food Chem. 2015 Jul 15;63(27):6133-41. doi: 10.1021/acs.jafc.5b01729. Epub 2015 Jun 29.

Abstract

Grass pea (Lathyrus sativus L.) cultivation is limited because of the presence in seeds and tissues of the nonprotein amino acid β-N-oxalyl-L-α,β-diaminopropionic acid (β-ODAP), a neurotoxin that can cause lathyrism in humans. Seven grass pea genotypes differing in seed β-ODAP concentration were grown in pots at three levels of water availability to follow changes in the concentration and amount of β-ODAP in leaves and pods and seeds. The concentration and amount of β-ODAP decreased in leaves in early reproductive development and in pods as they matured, while water stress increased β-ODAP concentration in leaves and pods at these stages. The net amount of β-ODAP in leaves and pods at early podding was positively associated with seed β-ODAP concentration at maturity. We conclude that variation among genotypes in seed β-ODAP concentration results from variation in net accumulation of β-ODAP in leaves and pods during vegetative and early reproductive development.

Keywords: reproductive phase; seed yield; vegetative phase; water stress; β-ODAP amount.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids, Diamino / analysis
  • Amino Acids, Diamino / metabolism*
  • Fruit / chemistry
  • Fruit / genetics
  • Fruit / growth & development*
  • Fruit / metabolism
  • Genotype
  • Lathyrus / chemistry
  • Lathyrus / genetics
  • Lathyrus / growth & development
  • Lathyrus / metabolism*
  • Neurotoxins / analysis
  • Neurotoxins / metabolism*
  • Plant Leaves / chemistry
  • Plant Leaves / genetics
  • Plant Leaves / growth & development*
  • Plant Leaves / metabolism
  • Seeds / chemistry
  • Seeds / genetics
  • Seeds / growth & development
  • Seeds / metabolism*
  • Water / analysis
  • Water / metabolism*

Substances

  • Amino Acids, Diamino
  • Neurotoxins
  • Water
  • oxalyldiaminopropionic acid