A large set of lowest and medium energy conformers of aliphatic tetramine trien was used to uncover structural-topological preferences of poliamines. Numerous common structural features among HL and H 2 L tautomers were identified, e.g., H-atoms of protonated functional groups are always involved in intramolecular NH•••N interactions and they result in as large and as many as possible rings in lowest energy conformers. Largest, 11-membered, molecular rings stabilize a molecule most and they appeared to be strain free whereas 5-memebred intramolecular rings were most strained (all formed due to NH•••N interactions). The CH•••HC interactions with QTAIM-defined atomic interaction lines were also found but, surprisingly, mainly in the lowest energy conformers of HL tautomers. According to the non-covalent interaction-based (NCI) analysis, 5-memebered rings formed by CH•••HC interactions are not strained and, in general, 3D NCI isosurfaces mimic those obtained for weaker NH•••N interactions. Also, 3D NCI isosurfaces found for NH•••N and CH•••HC interactions, regardless whether linked or not by an atomic interaction line, appeared to be indistinguishable. Using lowest energy conformers, theoretically predicted mixture of primary (HL p ) and secondary (HL s ) forms of trien was found to be in accord with the literature reports; using linear conformers resulted in predicting HL s as the only tautomer formed. In contrast to HF, the overall performance of B3LYP was found satisfactory for the purpose of the study.