Abstract
A cationic star copolymer with a β-cyclodextrin core was developed for nonviral gene transfer to mouse embryonic stem cells (mESCs). The copolymer comprises poly(2-dimethyl aminoethyl methacrylate) as the cationic component and poly(2-hydroxyethyl methacrylate) as the non-toxic stealth component. These materials have very low toxicity and show highly efficient transfection to mESC colonies.
MeSH terms
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Animals
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Cations / chemistry
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Mice
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Microscopy, Fluorescence
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Mouse Embryonic Stem Cells / cytology
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Mouse Embryonic Stem Cells / metabolism*
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Plasmids / genetics
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Plasmids / metabolism
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Polymers / chemistry*
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Transfection
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beta-Cyclodextrins / chemistry*
Substances
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Cations
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Polymers
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beta-Cyclodextrins
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enhanced green fluorescent protein
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Green Fluorescent Proteins