Down-regulation of GPX3 is associated with favorable/intermediate karyotypes in de novo acute myeloid leukemia

Int J Clin Exp Pathol. 2015 Mar 1;8(3):2384-91. eCollection 2015.

Abstract

Decreased glutathione peroxidase 3 (GPX3) expression has been identified in numerous solid tumors. However, GPX3 expression pattern in acute myeloid leukemia (AML) remains poorly known. Our study was intended to explore GPX3 expression status and further analyze the clinical relevance of GPX3 expression in AML. GPX3 mRNA level was detected by real-time quantitative PCR in 122 de novo AML patients and 44 normal controls. GPX3 transcript level was significantly decreased compared with normal controls (P<0.001). The patients with low GPX3 expression had significantly higher hemoglobin and platelets than those with high GPX3 expression (P=0.049 and 0.020). The frequency of low GPX3 expression in favorable karyotype (66%, 23/35) and intermediate karyotype (65%, 45/69) was higher than in poor karyotype (29%, 4/14) (P=0.017). No significant differences were observed in both complete remission and overall survival between the GPX3 low-expressed and high-expressed patients (P>0.05). Reduced GPX3 expression is associated with favorable/intermediate karyotypes but not with survival in de novo AML patients.

Keywords: GPX3; acute myeliod leukemia; expression; karyotype.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / genetics*
  • Biomarkers, Tumor / metabolism
  • Case-Control Studies
  • Child
  • Down-Regulation
  • Female
  • Genetic Predisposition to Disease
  • Glutathione Peroxidase / genetics*
  • Glutathione Peroxidase / metabolism
  • Hemoglobins / analysis
  • Humans
  • K562 Cells
  • Kaplan-Meier Estimate
  • Karyotype*
  • Karyotyping
  • Leukemia, Myeloid, Acute / blood
  • Leukemia, Myeloid, Acute / enzymology
  • Leukemia, Myeloid, Acute / genetics*
  • Leukemia, Myeloid, Acute / mortality
  • Male
  • Middle Aged
  • Phenotype
  • Platelet Count
  • Predictive Value of Tests
  • Prognosis
  • RNA, Messenger / genetics
  • Real-Time Polymerase Chain Reaction
  • Risk Factors
  • Time Factors
  • Young Adult

Substances

  • Biomarkers, Tumor
  • Hemoglobins
  • RNA, Messenger
  • GPX3 protein, human
  • Glutathione Peroxidase