Synthesis and in vitro anti-cancer evaluation of luteinizing hormone-releasing hormone-conjugated peptide

Amino Acids. 2015 Nov;47(11):2359-66. doi: 10.1007/s00726-015-2021-2. Epub 2015 Jun 10.

Abstract

Luteinizing hormone-releasing hormone (LHRH) is a decapeptide hormone released from the hypothalamus and shows high affinity binding to the LHRH receptors. It is reported that several cancer cells also express LHRH receptors such as breast, ovarian, prostatic, bladder and others. In this study, we linked B1, an anti-cancer peptide, to LHRH and its analogs to improve the activity against cancer cells with LHRH receptor. Biological evaluation revealed that TB1, the peptide contains triptorelin sequence, present favorable anti-cancer activity as well as plasma stability. Further investigations disclosed that TB1 trigger apoptosis by activating the mitochondria-cytochrome c-caspase apoptotic pathway, it also exhibited the anti-migratory effect on cancer cells.

Keywords: Anti-cancer; Apoptosis; Conjugation; Luteinizing hormone-releasing hormone; Peptide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents* / chemical synthesis
  • Antineoplastic Agents* / chemistry
  • Antineoplastic Agents* / pharmacology
  • Apoptosis / drug effects*
  • Drug Screening Assays, Antitumor
  • Female
  • Gonadotropin-Releasing Hormone* / chemical synthesis
  • Gonadotropin-Releasing Hormone* / chemistry
  • Gonadotropin-Releasing Hormone* / pharmacology
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • K562 Cells
  • MCF-7 Cells
  • Male
  • Neoplasms / drug therapy*
  • Neoplasms / metabolism
  • Neoplasms / pathology
  • Rabbits

Substances

  • Antineoplastic Agents
  • Gonadotropin-Releasing Hormone