Characterization of Two Human Monoclonal Antibodies Neutralizing Influenza A H7N9 Viruses

Jianmin Wang; Zhe Chen; Linlin Bao; Weijia Zhang; Ying Xue; XingHuo Pang; Xi Zhang; Chuan Qin; Qi Jin

doi:10.1128/JVI.01295-15

Characterization of Two Human Monoclonal Antibodies Neutralizing Influenza A H7N9 Viruses

J Virol. 2015 Sep;89(17):9115-8. doi: 10.1128/JVI.01295-15. Epub 2015 Jun 10.

Authors

Jianmin Wang¹, Zhe Chen¹, Linlin Bao², Weijia Zhang¹, Ying Xue¹, XingHuo Pang³, Xi Zhang⁴, Chuan Qin⁵, Qi Jin⁶

Affiliations

¹ MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
² Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Comparative Medicine Center, Peking Union Medical College (PUMC), Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Beijing, China.
³ Beijing Municipal Center for Disease Control and Prevention, Beijing, China.
⁴ Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China.
⁵ Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Comparative Medicine Center, Peking Union Medical College (PUMC), Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Beijing, China [email protected] [email protected].
⁶ MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing, China [email protected] [email protected].

Abstract

H7N9 was a cause of significant global health concern due to its severe infection and approximately 35% mortality in humans. By screening a Fab antibody phage library derived from patients who recovered from H7N9 infections, we characterized two human monoclonal antibodies (HuMAbs), HNIgGD5 and HNIgGH8. The epitope of these two antibodies was dependent on two residues in the receptor binding site at positions V186 and L226 of the hemagglutinin glycoprotein. Both antibodies possessed high neutralizing activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / immunology*
Antibodies, Neutralizing / immunology
Antibodies, Viral / immunology*
Binding Sites / immunology
Epitopes / immunology
Hemagglutinin Glycoproteins, Influenza Virus / immunology*
Humans
Influenza A Virus, H7N9 Subtype / immunology*
Influenza, Human
Mice
Mice, Inbred BALB C
Neutralization Tests
Orthomyxoviridae Infections / immunology
Protein Binding / immunology
Receptors, Virus / immunology*

Substances

Antibodies, Monoclonal
Antibodies, Neutralizing
Antibodies, Viral
Epitopes
Hemagglutinin Glycoproteins, Influenza Virus
Receptors, Virus
hemagglutinin, human influenza A virus