The complete mitochondrial genome sequence of Red knobby newt Tylototriton shanjing (Amphibia: Caudata)

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2773-4. doi: 10.3109/19401736.2015.1053059. Epub 2015 Jun 11.

Abstract

The complete mitogenome of Tylototriton shanjing is 16,661 bp in length with GenBank accession number KR154461, which contains 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNA), 2 ribosomal RNA genes (rRNA), and 1 control region (CR). The overall base composition of this mitogenome is biased toward AT content at 59.45%. Most of the PCGs and tRNA genes are located on the H-strand, except for ND6 subunit gene and eight tRNA genes, which were distributed on the L-strand. The PCGs used "ATG" and "GTG" as the start codons, while "TAA", "TAG", "AGA", and "T-" are used as stop codons. Almost all tRNA genes were folded into typical cloverleaf secondary structures. The T. shanjing genome had two tandem repeat sequences in the cob-noncoding region. The mitogenomic phylogenetic analyses shows that the genera Echinotriton and Tylototriton were clustered into a strong supported monophyletic clade, which is a sister clade to the genus Pleurodeles, this confirms the previous phylogenetic results.

Keywords: Caudata; Salamandridae; Tylototriton shanjing; mitochondrial genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Composition / genetics
  • Genome, Mitochondrial / genetics*
  • Phylogeny
  • RNA, Ribosomal / genetics
  • RNA, Transfer / genetics
  • Salamandridae / classification
  • Salamandridae / genetics*
  • Sequence Analysis, DNA

Substances

  • RNA, Ribosomal
  • RNA, Transfer