Abstract
A chemoenzymatic approach for synthesizing heparan sulfate oligosaccharides with a reactive diazoacetyl saccharide residue is reported. The resultant oligosaccharides were demonstrated to serve as specific inhibitors for heparan sulfate sulfotransferases, offering a new set of tools to probe the structural selectivity for heparan sulfate-binding proteins.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Enzyme Inhibitors / chemical synthesis*
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / pharmacology
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Heparitin Sulfate / chemical synthesis*
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Heparitin Sulfate / chemistry
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Heparitin Sulfate / pharmacology
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Molecular Probes / chemical synthesis*
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Molecular Probes / chemistry
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Molecular Probes / pharmacology
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Molecular Structure
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Structure-Activity Relationship
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Sulfotransferases / antagonists & inhibitors
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Sulfotransferases / metabolism
Substances
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Enzyme Inhibitors
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Molecular Probes
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Heparitin Sulfate
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Sulfotransferases
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heparitin sulfotransferase