Interactions of Isophorone Derivatives with DNA: Spectroscopic Studies

Marco Deiana; Katarzyna Matczyszyn; Julien Massin; Joanna Olesiak-Banska; Chantal Andraud; Marek Samoc

doi:10.1371/journal.pone.0129817

Interactions of Isophorone Derivatives with DNA: Spectroscopic Studies

PLoS One. 2015 Jun 12;10(6):e0129817. doi: 10.1371/journal.pone.0129817. eCollection 2015.

Authors

Marco Deiana¹, Katarzyna Matczyszyn¹, Julien Massin², Joanna Olesiak-Banska¹, Chantal Andraud², Marek Samoc¹

Affiliations

¹ Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Technology, Wyb. Wyspianskiego 27, 50-370, Wroclaw, Poland.
² Laboratoire de Chimie, CNRS UMR 5182, Ecole Normale Supérieure de Lyon, Université Lyon 1, Lyon, France.

Abstract

Interactions of three new isophorone derivatives, Isoa Isob and Isoc with salmon testes DNA have been investigated using UV-Vis, fluorescence and circular dichroism spectroscopic methods. All the studied compounds interact with DNA through intercalative binding mode. The stoichiometry of the isophorone/DNA adducts was found to be 1:1. The fluorescence quenching data revealed a binding interaction with the base pairs of DNA. The CD data indicate that all the investigated isophorones induce DNA modifications.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cyclohexanones / chemistry*
DNA / chemistry*
Intercalating Agents / chemistry*

Substances

Cyclohexanones
Intercalating Agents
isophorone
DNA

Grants and funding

The financing from NCN Opus grant DEC-2013/09/B/ST5/03417 is highly acknowledged. The authors thank the Région Rhône-Alpes (cluster5) for a grant to J.M. and financial support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.