Genome-derived cytosolic DNA contributes to type I interferon expression and immunogenicity of B-cell lymphoma cells

Yu J Shen; Samantha S W Ho; Nikki Y Tan; Christine Xing'Er Koo; Muznah Khatoo; Florence S G Cheung; Stephan Gasser

doi:10.1016/j.cyto.2015.05.024

Genome-derived cytosolic DNA contributes to type I interferon expression and immunogenicity of B-cell lymphoma cells

Cytokine. 2015 Dec;76(2):581-582. doi: 10.1016/j.cyto.2015.05.024. Epub 2015 Jun 9.

Authors

Yu J Shen¹, Samantha S W Ho², Nikki Y Tan², Christine Xing'Er Koo¹, Muznah Khatoo², Florence S G Cheung², Stephan Gasser³

Affiliations

¹ Immunology Programme and Department of Microbiology, Centre for Life Science, National University of Singapore, 117456, Singapore; NUS Graduate School for Integrative Sciences & Engineering, National University of Singapore, 117456, Singapore.
² Immunology Programme and Department of Microbiology, Centre for Life Science, National University of Singapore, 117456, Singapore.
³ Immunology Programme and Department of Microbiology, Centre for Life Science, National University of Singapore, 117456, Singapore; NUS Graduate School for Integrative Sciences & Engineering, National University of Singapore, 117456, Singapore. Electronic address: [email protected].

PMID: 26070935
DOI: 10.1016/j.cyto.2015.05.024

Abstract

We recently provided evidence that genome-derived DNA is present in the cytosol of many tumor cells. Genomic loci that give rise to cytosolic DNA can potentially form non-B DNA structures including triple-stranded RNA:DNA structures (R-loops). The RNA:DNA-specific endonuclease RNaseh1 reduced the levels of cytosolic DNA and type I interferon-dependent rejection of B-cell lymphoma suggesting that cytosolic DNA may contribute to immune surveillance of B-cell lymphoma.

Keywords: Cancer; DNA sensors; Innate immunity; Non-B DNA motifs; Type I interferon.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
DNA / genetics*
Genome*
Humans
Interferon Type I / genetics*
Lymphoma, B-Cell / genetics*
Lymphoma, B-Cell / immunology*
RNA / genetics

Substances

Interferon Type I
RNA
DNA