Generation of iPSC line HEL47.2 from healthy human adult fibroblasts

Ras Trokovic; Jere Weltner; Timo Otonkoski

doi:10.1016/j.scr.2015.05.013

Generation of iPSC line HEL47.2 from healthy human adult fibroblasts

Stem Cell Res. 2015 Jul;15(1):263-5. doi: 10.1016/j.scr.2015.05.013. Epub 2015 Jun 2.

Authors

Ras Trokovic¹, Jere Weltner², Timo Otonkoski³

Affiliations

¹ Research Programs Unit, Molecular Neurology, Biomedicum Stem Cell Centre, University of Helsinki, Helsinki, Finland. Electronic address: [email protected].
² Research Programs Unit, Molecular Neurology, Biomedicum Stem Cell Centre, University of Helsinki, Helsinki, Finland.
³ Research Programs Unit, Molecular Neurology, Biomedicum Stem Cell Centre, University of Helsinki, Helsinki, Finland; Children's Hospital, Helsinki University Central Hospital, Helsinki, Finland.

PMID: 26096151
DOI: 10.1016/j.scr.2015.05.013

Abstract

Human iPSC line HEL47.2 was generated from healthy 83-year old male dermal fibroblasts using non-integrative reprogramming method. Reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using Sendai viruses.

MeSH terms

Adult
Aged, 80 and over
Cell Line / cytology*
Cellular Reprogramming / drug effects
Fibroblasts / cytology*
Fibroblasts / drug effects
Foreskin / cytology
Health*
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / drug effects
Kruppel-Like Factor 4
Male
Transcription Factors / pharmacology

Substances

KLF4 protein, human
Kruppel-Like Factor 4
Transcription Factors