Hybridization chain reaction engineered dsDNA for Cu metallization: an enzyme-free platform for amplified detection of cancer cells and microRNAs

Chem Commun (Camb). 2015 Jul 21;51(57):11496-9. doi: 10.1039/c5cc03144c.

Abstract

A novel enzyme-free platform for amplified detection of cancer cells and miRNAs was constructed with high sensitivity by fluorescent Cu metallization on HCR engineered dsDNA templates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aptamers, Nucleotide / chemistry
  • Copper / chemistry*
  • DNA / chemistry*
  • Fluorescent Dyes / chemistry*
  • Hep G2 Cells
  • Humans
  • MCF-7 Cells
  • Mice
  • MicroRNAs / analysis*
  • NIH 3T3 Cells
  • Nanoparticles / chemistry*
  • Nanoparticles / ultrastructure
  • Neoplasms / diagnosis*
  • Nucleic Acid Hybridization / methods*
  • Spectrometry, Fluorescence / methods

Substances

  • Aptamers, Nucleotide
  • Fluorescent Dyes
  • MicroRNAs
  • Copper
  • DNA