Abstract
We apply a quantum diamond microscope for detection and imaging of immunomagnetically labeled cells. This instrument uses nitrogen-vacancy (NV) centers in diamond for correlated magnetic and fluorescence imaging. Our device provides single-cell resolution and a field of view (∼1 mm(2)) two orders of magnitude larger than that of previous NV imaging technologies, enabling practical applications. To illustrate, we quantified cancer biomarkers expressed by rare tumor cells in a large population of healthy cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Antibodies / chemistry
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Biomarkers, Tumor
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Cell Line, Tumor
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Diagnostic Imaging / methods
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Diamond
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Humans
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Image Processing, Computer-Assisted / methods*
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MCF-7 Cells
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Magnetic Phenomena*
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Magnetics
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Microscopy / instrumentation*
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Microscopy / methods
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Microscopy, Fluorescence
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Nanotechnology / methods
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Nitrogen / chemistry
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Quantum Theory
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Single-Cell Analysis*
Substances
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Antibodies
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Biomarkers, Tumor
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Diamond
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Nitrogen