Integration of DNA bio-gates and duplex-specific nuclease signal amplification: towards electrochemiluminescence detection of survivin mRNA

Chem Commun (Camb). 2015 Jul 25;51(58):11673-6. doi: 10.1039/c5cc03693c. Epub 2015 Jun 23.

Abstract

Using the DNA bio-gate and duplex-specific nuclease assisted target recycling, a facile electrochemiluminescence assay was developed for the sensitive detection of survivin mRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Line, Tumor
  • DNA / chemistry
  • Deoxyribonucleases / chemistry
  • Humans
  • Inhibitor of Apoptosis Proteins / genetics*
  • Luminescence
  • Nanoparticles / classification
  • Organometallic Compounds / chemistry
  • RNA, Messenger / analysis*
  • RNA, Messenger / chemistry
  • Silicon Dioxide / chemistry
  • Survivin

Substances

  • BIRC5 protein, human
  • Inhibitor of Apoptosis Proteins
  • Organometallic Compounds
  • RNA, Messenger
  • Survivin
  • tris(2,2'-bipyridyl)ruthenium(II)
  • Silicon Dioxide
  • DNA
  • Deoxyribonucleases