Distinct clinicopathological phenotype of hepatocellular carcinoma with ethoxybenzyl-magnetic resonance imaging hyperintensity: association with gene expression signature

Am J Surg. 2015 Sep;210(3):561-9. doi: 10.1016/j.amjsurg.2015.03.027. Epub 2015 Jun 1.

Abstract

Background: Although hepatocellular carcinoma (HCC) is mostly a lower intensity lesion in the hepatobiliary phase on gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid-enhanced magnetic resonance imaging, some HCCs were shown as a higher intensity lesion (high HCC). This study aimed to reveal the clinicopathological and biological properties of high HCC.

Methods: Patients who underwent curative hepatectomy as the first treatment for HCC were included. HCC was defined as high HCC if the ratio between the signal intensity of the HCC and the background liver was greater than or equal to 1.0. We retrospectively performed clinicopathological and global gene expression analyses.

Results: Of the 77 patients, 14 had high HCC. Serum protein induced by vitamin K absence or antagonist II levels in high HCC were lower, and the high HCCs were well differentiated. The 3-year disease-free survival rates in high HCC and low HCC patients were 90% and 54%, respectively (P = .035). Overall survival did not differ significantly. Global gene expression analysis revealed that SLCO1B3 was upregulated in high HCC.

Conclusions: Clinicopathological analysis revealed low-grade malignancy in high HCCs compared with low HCCs. The expression of SLCO1B3 was key to the hyperintensity in the hepatobiliary phase of ethoxybenzyl-diethylenetriamine pentaacetic acid magnetic resonance imaging.

Keywords: EOB-MRI; Gd-EOB-DTPA-enhanced MRI; Hepatocellular carcinoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Biomarkers / blood
  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Hepatocellular / mortality
  • Carcinoma, Hepatocellular / pathology*
  • Carcinoma, Hepatocellular / surgery
  • Contrast Media
  • Disease-Free Survival
  • Female
  • Gadolinium DTPA
  • Gene Expression*
  • Humans
  • Image Enhancement / methods*
  • Liver Neoplasms / genetics*
  • Liver Neoplasms / mortality
  • Liver Neoplasms / pathology*
  • Liver Neoplasms / surgery
  • Magnetic Resonance Imaging
  • Male
  • Neoplasm Invasiveness
  • Organic Anion Transporters, Sodium-Independent / genetics
  • Organic Anion Transporters, Sodium-Independent / metabolism
  • Protein Precursors / blood
  • Prothrombin
  • Retrospective Studies
  • Solute Carrier Organic Anion Transporter Family Member 1B3
  • Up-Regulation

Substances

  • Biomarkers
  • Contrast Media
  • Organic Anion Transporters, Sodium-Independent
  • Protein Precursors
  • SLCO1B3 protein, human
  • Solute Carrier Organic Anion Transporter Family Member 1B3
  • gadolinium ethoxybenzyl DTPA
  • acarboxyprothrombin
  • Prothrombin
  • Gadolinium DTPA