Prediction of development and maintenance of high albuminuria during chronic renin-angiotensin suppression by plasma proteomics

Int J Cardiol. 2015 Oct 1:196:170-7. doi: 10.1016/j.ijcard.2015.05.148. Epub 2015 May 29.

Abstract

Background: High albuminuria is a strong predictor of development of cardiovascular events in hypertensive patients. The search for predictors identifying patients at risk of developing high albuminuria or presenting a more rapid progression in this parameter may represent an effective strategy for adequate intervention and better outcome.

Methods and results: Initially we investigated 24 patients presenting with normoalbuminuria, de novo albuminuria and sustained albuminuria. Plasma proteomics disclosed an upregulation of ceruloplasmin (CP), haptoglobin (HP) and alpha 1-acid glycoprotein (ORM1) that in a second step were selected for validation using turbidimetry assay in a cohort of 105 subjects. The validation showed that HP and ORM1 proteins were increased in patients presenting with very high albuminuria and potential irreversible kidney damage. CP and HP correlated positively with albuminuria values in normoalbuminuric patients. Finally, the levels of ORM1 and CP were increased in patients who progressed in their levels of albuminuria.

Conclusions: Our findings show that these proteins may potentially be useful for predicting the development of high albuminuria and to monitor renal damage.

Keywords: Albuminuria; Hypertension; Organ damage; Plasma proteomics; RAS suppression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Albuminuria / blood
  • Albuminuria / diagnosis*
  • Biomarkers / blood*
  • Ceruloplasmin / metabolism*
  • Disease Progression
  • Female
  • Haptoglobins / metabolism
  • Humans
  • Hypertension / complications*
  • Kidney / injuries
  • Male
  • Middle Aged
  • Nephelometry and Turbidimetry
  • Orosomucoid / metabolism*
  • Proteomics / methods*
  • Renin-Angiotensin System / drug effects*
  • Up-Regulation

Substances

  • Biomarkers
  • Haptoglobins
  • Orosomucoid
  • Ceruloplasmin