Abstract
A mitogenic response to transforming growth factor beta (TGF) occurred in the UMR106 cells cultured in serum-free medium and exposed serially to estradiol and TGF. This mitogenic response was lost when insulin was removed from the medium. TGF inhibited growth and increased the alkaline phosphatase content in the UMR106 cells cultured in medium lacking insulin. Prior exposure of the cells to estradiol enhanced this response. Monoclonal antibodies against TGF blocked the estradiol induced inhibition of growth after a two day incubation in medium devoid of insulin.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alkaline Phosphatase / metabolism
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Animals
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Cell Division / drug effects
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Estradiol / pharmacology
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Estrogens / pharmacology*
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Osteoblasts / cytology*
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Osteoblasts / enzymology
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Osteosarcoma / enzymology
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Osteosarcoma / pathology
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Rats
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Transforming Growth Factors / pharmacology*
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Transforming Growth Factors / physiology
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / enzymology
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Tumor Cells, Cultured / pathology
Substances
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Estrogens
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Estradiol
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Transforming Growth Factors
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Alkaline Phosphatase