Abstract
Here, we report a quick and low-cost method to improve plant transformation using Agrobacterium tumefaciens. This method involves the use of physical wounding, ultrasound, and an increase in exposure time to the bacteria. We show how the transformation rate increased from 0 to 14% when an ultrasound pulse of 10 s was used in conjunction with 96 h of bacterial exposure in Eclipta alba explants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Agrobacterium tumefaciens / genetics*
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Agrobacterium tumefaciens / metabolism
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Anti-Bacterial Agents / pharmacology
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DNA, Bacterial / genetics*
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DNA, Bacterial / metabolism
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Eclipta / drug effects
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Eclipta / genetics*
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Eclipta / microbiology
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Eclipta / radiation effects
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Genetic Vectors / chemistry
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Genetic Vectors / metabolism
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Kanamycin / pharmacology
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Kanamycin Resistance
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Plant Stems / drug effects
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Plant Stems / genetics*
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Plant Stems / microbiology
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Plant Stems / radiation effects
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Plants, Genetically Modified*
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Transformation, Genetic*
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Ultrasonic Waves
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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Kanamycin