Objective: To observe the cytotoxity of CD138-CAR-T cells on human multiple myeloma cell RPMI8226 and U266 cells and explore the impact of pomalidomide on the cytotoxity of CD138-CAR-T on RPMI8226 and U266 cells.
Methods: The cytotoxity of CD138-CAR-T and CD138-CAR-T combined pomalidomide on RPMI8226 and U266 was detected by CFSE/7AAD. The effctor cells were co-cultured with target cells at 5:1 for 18 h, and then the supernatant were collected and used for ELISA assays.
Results: After 18 h co-culture, the cytotoxity of CD138-CAR-T on RPMI8226 and U266 was significantiy higher than control (P<0.01). There was no significant change on the cytotoxity of pomalidoide combined with CD138-CAR-T on RPMI8226 and U266. The results showed that co-cultured system contribted to a markedly increased production of IFN-γ, after adding pomalidomide to the co-cultured system. It can significantly enhance the production of IFN-γ, compared with CD138-CAR-T alone.
Conclusion: CD138-CAR-T had significantly cytotoxity on U266 and RPMI8226. Pomalidomide could promote CD138-CAR-T cells IFN-γ production.
目的: 观察CD138-CAR-T细胞对人多发性骨髓瘤（MM）细胞株RPMI8226和U266细胞的杀伤作用，探讨泊马度胺对CD138-CAR-T细胞及其杀伤作用的影响。
方法: 采用CFSE/7-AAD双标法检测CD138-CAR-T细胞及其联合泊马度胺对RPMI8226、U266细胞的杀伤活性。ELISA法检测CD138-CAR-T细胞分泌IFN-γ的变化。
结果: CD138-CAR-T细胞作用18 h后，对RPMI8226、U266细胞的杀伤活性分别为（55.2±3.9）%、（85.1±2.4）%，对照组分别为（7.0±1.5）%、（12.5±2.1）%，差异均有统计学意义（P值均<0.01）；与CD138-CAR-T细胞组比较，CD138-CAR-T细胞联合泊马度胺（2.5 µg/ml）作用18 h后，对RPMI8226、U266细胞的杀伤活性差异无统计学意义（P值均>0.05）。与CD138-CAR-T细胞组比较，CD138-CAR-T细胞和MM细胞共培养组IFN-γ分泌水平显著增高；与共培养组比较，加入泊马度胺后能显著促进IFN-γ的释放，差异均有统计学意义（P值均<0.01）。
结论: CD138-CAR-T细胞对MM细胞及耐药细胞株均有明显的杀伤作用；其与MM细胞共培养能促进后者IFN-γ的分泌；泊马度胺能促进CD138-CAR-T细胞分泌IFN-γ。