BTB-ZF Protein Znf131 Regulates Cell Growth of Developing and Mature T Cells

Tomohiro Iguchi; Kazuhisa Aoki; Tomokatsu Ikawa; Masato Taoka; Choji Taya; Hiroshi Yoshitani; Makiko Toma-Hirano; Osamu Koiwai; Toshiaki Isobe; Hiroshi Kawamoto; Hisao Masai; Shoichiro Miyatake

doi:10.4049/jimmunol.1500602

BTB-ZF Protein Znf131 Regulates Cell Growth of Developing and Mature T Cells

J Immunol. 2015 Aug 1;195(3):982-93. doi: 10.4049/jimmunol.1500602. Epub 2015 Jul 1.

Authors

Affiliations

¹ Laboratory of Self Defense Gene Regulation, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan; Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda 278-8510, Japan;
² Laboratory of Self Defense Gene Regulation, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan;
³ Young Chief Investigators Laboratory for Immune Regeneration, RIKEN Center for Integrative Medical Sciences, Yokohama 230-0045, Japan;
⁴ Laboratory of Biochemistry, Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan;
⁵ Animal Research Division, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan;
⁶ Department of Otolaryngology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-8655, Japan;
⁷ Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda 278-8510, Japan;
⁸ Department of Immunology, Field of Regeneration Control, Institute of Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan; and.
⁹ Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan.
¹⁰ Laboratory of Self Defense Gene Regulation, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan; [email protected].

PMID: 26136427
DOI: 10.4049/jimmunol.1500602

Abstract

Many members of the BTB-ZF family have been shown to play important roles in lymphocyte development and function. The role of zinc finger Znf131 (also known as Zbtb35) in T cell lineage was elucidated through the production of mice with floxed allele to disrupt at different stages of development. In this article, we present that Znf131 is critical for T cell development during double-negative to double-positive stage, with which significant cell expansion triggered by the pre-TCR signal is coupled. In mature T cells, Znf131 is required for the activation of effector genes, as well as robust proliferation induced upon TCR signal. One of the cyclin-dependent kinase inhibitors, p21(Cip1) encoded by cdkn1a gene, is one of the targets of Znf131. The regulation of T cell proliferation by Znf131 is in part attributed to its suppression on the expression of p21(Cip1).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3 Cells
Animals
Cell Differentiation / immunology
Cell Line
Cell Proliferation
Cyclin-Dependent Kinase Inhibitor p21 / biosynthesis
Cyclin-Dependent Kinase Inhibitor p21 / genetics*
DNA-Binding Proteins / genetics
DNA-Binding Proteins / immunology*
Lymphocyte Activation / genetics
Lymphocyte Activation / immunology
Mice
Mice, Inbred C57BL
Mice, Transgenic
Nuclear Proteins / immunology
Promoter Regions, Genetic / genetics
Protein Inhibitors of Activated STAT / immunology
Proto-Oncogene Proteins c-myb / genetics
Proto-Oncogene Proteins c-myb / immunology
Receptors, Antigen, T-Cell, alpha-beta / genetics
Receptors, Antigen, T-Cell, alpha-beta / immunology*
T-Lymphocytes / cytology
T-Lymphocytes / immunology*
Transcription Factors / genetics
Transcription Factors / immunology*
Ubiquitin-Protein Ligases

Substances

Cdkn1a protein, mouse
Cyclin-Dependent Kinase Inhibitor p21
DNA-Binding Proteins
Nuclear Proteins
Protein Inhibitors of Activated STAT
Proto-Oncogene Proteins c-myb
Receptors, Antigen, T-Cell, alpha-beta
Transcription Factors
ZNF131 protein, mouse
Miz1 protein, mouse
Ubiquitin-Protein Ligases