Three blocks are not enough--Blocking of the murine IgG receptor FcγRIV is crucial for proper characterization of cells by FACS analysis

Eur J Immunol. 2015 Sep;45(9):2694-7. doi: 10.1002/eji.201545463. Epub 2015 Jul 21.
No abstract available

Keywords: Antibodies; Blocking; Flow cytometry; Immunoglobulin receptors/Fc receptors; Immunological assays · Monocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Neutralizing / chemistry
  • Antibodies, Neutralizing / pharmacology
  • CHO Cells
  • Cricetulus
  • False Positive Reactions
  • Flow Cytometry / standards*
  • Gene Expression
  • Goats
  • Immunoglobulin G / classification
  • Immunoglobulin G / genetics
  • Immunoglobulin G / immunology*
  • Immunophenotyping / standards*
  • Killer Cells, Natural / cytology
  • Killer Cells, Natural / drug effects
  • Killer Cells, Natural / immunology
  • Mice
  • Monocytes / cytology
  • Monocytes / drug effects
  • Monocytes / immunology
  • Protein Isoforms / antagonists & inhibitors
  • Protein Isoforms / genetics
  • Protein Isoforms / immunology
  • Rats
  • Receptors, IgG / antagonists & inhibitors*
  • Receptors, IgG / genetics
  • Receptors, IgG / immunology

Substances

  • Antibodies, Neutralizing
  • Fcgr4 protein, mouse
  • Immunoglobulin G
  • Protein Isoforms
  • Receptors, IgG