Ion torrent next-generation sequencing reveals the complete mitochondrial genome of endangered mahseer Tor khudree (Sykes, 1839)

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2949-50. doi: 10.3109/19401736.2015.1060455. Epub 2015 Jul 8.

Abstract

The complete mitochondrial genome of an endangered mahseer (Deccan mahseer), Tor khudree was sequenced using Ion torrent platform for the first time. The genome sequence was 16 573 bp in size, and consists of 13 protein coding genes, 22 tRNAs, 2 rRNA genes and 1 control region. The gene organization and its order were similar to other vertebrates. The overall base composition was A: 31.9%, G: 15.6%, C: 27.68%, T: 24.76%, A + T content 56.6% and the G + C content 43.32%. The phylogenetic tree constructed using a maximum likelihood model showed sister relationship between T. khudree and Tor tambroides.

Keywords: Mitogenome; next generation sequencing; phylogeny.

MeSH terms

  • Animals
  • Base Composition
  • Endangered Species*
  • Fishes / classification*
  • Fishes / genetics*
  • Genes, Mitochondrial
  • Genome Size
  • Genome, Mitochondrial*
  • High-Throughput Nucleotide Sequencing
  • Open Reading Frames
  • Phylogeny
  • Sequence Analysis, DNA
  • Whole Genome Sequencing