Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of Parkinsonism

Behav Neurol. 2015:2015:725897. doi: 10.1155/2015/725897. Epub 2015 Jun 16.

Abstract

The study has investigated the effect of isoflavone attenuates the caspase-1 and caspase-3 level in cell model of Parkinsonism. The subjects were PC12 cells. They were randomly divided into six groups: control, MPP(+) (250 μmol/L), isoflavone (10 μM), isoflavone (10 μM) + MPP(+) (250 μmol/L), Z-YVAD-CHO (10 nM) + MPP(+) group, and Z-DEVD-CHO (10 nM) + MPP(+) group. Cell viability was measured by MTT methods; the content of tyrosine hydroxylase was measured by immunocytochemistry method of avidinbiotin peroxidase complex; apoptosis ratio was measured by flow cytometry. The results showed that cell viability in the MPP(+) group was lower than in all other five groups. There was no difference in cell viability between isoflavone + MPP(+) and control group. Optical density of TH positive cells in isoflavone group was higher than in control, isoflavone + MPP(+), and MPP(+) only groups. The apoptosis ratio in the isoflavone + MPP(+) group and control group and the Z-YVAD-CHO + MPP(+) and Z-DEVD-CHO + MPP(+) group was similar, which was lower than in the MPP(+) group. The lowest apoptosis ratio was found in the isoflavone only group.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects*
  • Caspase 1 / metabolism*
  • Caspase 3 / metabolism*
  • Cell Survival / drug effects
  • Cells, Cultured
  • Isoflavones / pharmacology*
  • Neuroprotective Agents / pharmacology*
  • PC12 Cells
  • Parkinsonian Disorders / drug therapy
  • Parkinsonian Disorders / enzymology*
  • Rats

Substances

  • Isoflavones
  • Neuroprotective Agents
  • Casp3 protein, rat
  • Caspase 3
  • Caspase 1