Cytokine modulation by IL-35 in mice with allergic rhinitis

Am J Rhinol Allergy. 2015 Jul-Aug;29(4):251-6. doi: 10.2500/ajra.2015.29.4188.

Abstract

Background: Interleukin (IL) 35 was recently identified as an additional anti-inflammatory and immunosuppressive cytokine. However, the role of IL-35 in allergic rhinitis is not well understood. The effect of IL-35 on other cytokines in allergic responses is also unclear.

Objective: To investigate, in mice with allergic rhinitis, the effect of IL-35 on other cytokines associated with allergic rhinitis.

Methods: A murine model of allergic rhinitis was established, and splenic cells were collected. Ovalbumin-specific allergic T-cell response was measured. The production of cytokines (T helper 1 interferon-gamma), Th2 (IL-4, IL-5, IL-13), Th17 (IL-17), IL-12 family (IL-12, IL-23, IL-27), IL-2, tumor necrosis factor-alpha, transforming growth factor-beta, and IL-10) stimulated with antigen was also measured in the presence or absence of IL-35.

Results: IL-35 significantly inhibited the ovalbumin-specific T-cell response. It also significantly reduced the production of IL-4, IL-5, IL-13, IL-17, IL-23, and TNF-alpha, and significantly increased the production of IL-2, IL-10, and IL-27.

Conclusion: This study showed that IL-35 inhibits allergic T-cell response and has the ability to modulate the production of IL-2, IL-4, IL-5, IL-10, IL-13, IL-17, IL-23, IL-27, and TNF-alpha in mice with allergic rhinitis. This study also indicated the possibility of a novel therapy with IL-35 for the control of allergic rhinitis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cytokines / metabolism
  • Disease Models, Animal
  • Immunosuppression Therapy*
  • Interleukins / genetics
  • Interleukins / metabolism*
  • Mice
  • Nasal Mucosa / metabolism
  • Rhinitis, Allergic / genetics
  • Rhinitis, Allergic / metabolism*
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Cytokines
  • Interleukins
  • Tumor Necrosis Factor-alpha
  • interleukin-35, mouse