Identification of a novel NDM variant, NDM-13, from a multidrug-resistant Escherichia coli clinical isolate in Nepal

Basudha Shrestha; Tatsuya Tada; Tohru Miyoshi-Akiyama; Kayo Shimada; Hiroshi Ohara; Teruo Kirikae; Bharat M Pokhrel

doi:10.1128/AAC.00332-15

Identification of a novel NDM variant, NDM-13, from a multidrug-resistant Escherichia coli clinical isolate in Nepal

Antimicrob Agents Chemother. 2015 Sep;59(9):5847-50. doi: 10.1128/AAC.00332-15. Epub 2015 Jul 13.

Authors

Basudha Shrestha¹, Tatsuya Tada², Tohru Miyoshi-Akiyama³, Kayo Shimada², Hiroshi Ohara⁴, Teruo Kirikae⁵, Bharat M Pokhrel¹

Affiliations

¹ Department of Microbiology, Institute of Medicine, Tribhuvan University, Maharajgunj, Kathmandu, Nepal.
² Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo, Japan.
³ Pathogenic Microbe Laboratory, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo, Japan.
⁴ Bureau of International Medical Cooperation, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo, Japan.
⁵ Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo, Japan [email protected].

Abstract

A novel New Delhi metallo-β-lactamase, NDM-13, was identified in a carbapenem-resistant Escherichia coli clinical isolate obtained from the urine of a patient in Nepal. The enzymatic activity of NDM-13 against β-lactams was similar to that of NDM-1. However, NDM-13 displayed significantly higher k cat/Km ratios for cefotaxime. The genetic environment of bla NDM-13 was determined to be tnpA-IS30-bla NDM-13-ble MBL-trpF-dsbC-cutA-groES-groL, with bla NDM-13 located within the chromosome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Cefotaxime / pharmacology
Drug Resistance, Multiple, Bacterial
Escherichia coli / drug effects*
Escherichia coli / enzymology
Microbial Sensitivity Tests
Nepal
beta-Lactams / pharmacology*

Substances

Anti-Bacterial Agents
beta-Lactams
Cefotaxime