2'-5'-Oligoadenylate Synthetase-Like Protein Inhibits Respiratory Syncytial Virus Replication and Is Targeted by the Viral Nonstructural Protein 1

Jayeeta Dhar; Rolando A Cuevas; Ramansu Goswami; Jianzhong Zhu; Saumendra N Sarkar; Sailen Barik

doi:10.1128/JVI.01076-15

2'-5'-Oligoadenylate Synthetase-Like Protein Inhibits Respiratory Syncytial Virus Replication and Is Targeted by the Viral Nonstructural Protein 1

J Virol. 2015 Oct;89(19):10115-9. doi: 10.1128/JVI.01076-15. Epub 2015 Jul 15.

Authors

Jayeeta Dhar¹, Rolando A Cuevas², Ramansu Goswami¹, Jianzhong Zhu², Saumendra N Sarkar³, Sailen Barik⁴

Affiliations

¹ Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University, Cleveland, Ohio, USA.
² Cancer Virology Program, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
³ Cancer Virology Program, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA [email protected] [email protected].
⁴ Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences, Cleveland State University, Cleveland, Ohio, USA [email protected] [email protected].

Abstract

2'-5'-Oligoadenylate synthetase-like protein (OASL) is an interferon-inducible antiviral protein. Here we describe differential inhibitory activities of human OASL and the two mouse OASL homologs against respiratory syncytial virus (RSV) replication. Interestingly, nonstructural protein 1 (NS1) of RSV promoted proteasome-dependent degradation of specific OASL isoforms. We conclude that OASL acts as a cellular antiviral protein and that RSV NS1 suppresses this function to evade cellular innate immunity and allow virus growth.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

2',5'-Oligoadenylate Synthetase / immunology
2',5'-Oligoadenylate Synthetase / physiology*
Animals
HEK293 Cells
Host-Pathogen Interactions
Humans
Immune Evasion
Immunity, Cellular
Mice
Proteasome Endopeptidase Complex / metabolism
Proteolysis
Respiratory Syncytial Virus Infections / immunology
Respiratory Syncytial Virus Infections / virology
Respiratory Syncytial Viruses / genetics
Respiratory Syncytial Viruses / immunology
Respiratory Syncytial Viruses / physiology*
Viral Nonstructural Proteins / genetics
Viral Nonstructural Proteins / physiology*
Virus Replication / immunology
Virus Replication / physiology*

Substances

NS2 protein, human respiratory syncytial virus
Viral Nonstructural Proteins
OASL protein, human
Oasl1 protein, mouse
Oasl2 protein, mouse
2',5'-Oligoadenylate Synthetase
Proteasome Endopeptidase Complex

Abstract

Publication types

MeSH terms

Substances

Grants and funding