Abstract
SIRT6, a member of the sirtuin family, has been identified as a candidate tumor suppressor. To pursue the role of SIRT6 in endometrial cancer, we investigated the anti-tumorigenic function of SIRT6. The expression of SIRT6 negatively affected the proliferation of AN3CA and KLE endometrial cancer cells. Increased expression of SIRT6 resulted in the induction of apoptosis by repressing the expression of the anti-apoptotic protein survivin. Consistent with this result, a survivin inhibitor YM155 efficiently inhibited cellular proliferation and induced apoptosis. These results revealed that SIRT6 might function as a tumor suppressor of endometrial cancer cells.
Keywords:
Apoptosis; Endometrial cancer; SIRT6; Survivin; YM155.
Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Apoptosis / drug effects
-
Apoptosis / genetics*
-
Blotting, Western
-
Cell Cycle / genetics
-
Cell Line, Tumor
-
Cell Proliferation / drug effects
-
Cell Proliferation / genetics*
-
Endometrial Neoplasms / genetics
-
Endometrial Neoplasms / metabolism
-
Endometrial Neoplasms / pathology
-
Female
-
Gene Expression Regulation, Neoplastic*
-
Humans
-
Imidazoles / pharmacology
-
Immunohistochemistry
-
Inhibitor of Apoptosis Proteins / antagonists & inhibitors
-
Inhibitor of Apoptosis Proteins / genetics
-
Inhibitor of Apoptosis Proteins / metabolism
-
Kaplan-Meier Estimate
-
Naphthoquinones / pharmacology
-
RNA Interference
-
Reverse Transcriptase Polymerase Chain Reaction
-
Sirtuins / genetics*
-
Sirtuins / metabolism
-
Survivin
-
Tumor Suppressor Proteins / genetics
-
Tumor Suppressor Proteins / metabolism
Substances
-
BIRC5 protein, human
-
Imidazoles
-
Inhibitor of Apoptosis Proteins
-
Naphthoquinones
-
Survivin
-
Tumor Suppressor Proteins
-
SIRT6 protein, human
-
Sirtuins
-
sepantronium