Apigenin Attenuates β-Receptor-Stimulated Myocardial Injury Via Safeguarding Cardiac Functions and Escalation of Antioxidant Defence System

Cardiovasc Toxicol. 2016 Jul;16(3):286-97. doi: 10.1007/s12012-015-9336-9.

Abstract

Apigenin (AP) is a flavone in dietary flavonoids reported as strong antioxidant and elite modulator of PPARγ. The current study evaluated the consequence of AP in isoproterenol (ISO)-induced oxidative stress and myocardial infarction during β-adrenergic receptor stimulus in rats by persistent hemodynamic, biochemical and histopathological changes. Rats received AP (25, 50 and 75 mg/kg/day) or vehicle i.p. for 14 days and ISO (100 mg/kg, s.c.) on 13th and 14th days for initiation of cardiotoxicity. ISO-treated rats showed evidence of significant dwindle in systolic and diastolic arterial pressures, maximal positive rate of developed left ventricular pressure. In totting up, a noteworthy diminution in activities of creatine kinase-MB isoenzyme, reduced glutathione, superoxide dismutase, catalase and level along with rise in malondialdehyde content were observed. The shielding function of AP on isoproterenol-induced myocardial damage was observed by attenuating all the endogenous parameters and the membrane-bound enzymes. It was confirmed by histopathological examinations. The effect of AP at the doses of 50 and 75 mg/kg showed added apparent than at the dose of 25 mg/kg. Current study thus provides confirmation for protective effects of AP on myocardium in experimentally induced myocardial infarction.

Keywords: Apigenin; Isoproterenol; Myocardial infarction; Myocardial necrosis; ROS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Apigenin / pharmacology*
  • Biomarkers / metabolism
  • Catalase / metabolism
  • Creatine Kinase, MB Form / metabolism
  • Cytoprotection
  • Disease Models, Animal
  • Glutathione / metabolism
  • Hemodynamics / drug effects
  • Isoproterenol*
  • Male
  • Malondialdehyde / metabolism
  • Myocardial Infarction / chemically induced
  • Myocardial Infarction / metabolism
  • Myocardial Infarction / pathology
  • Myocardial Infarction / prevention & control*
  • Myocardium / metabolism*
  • Myocardium / pathology
  • Necrosis
  • Oxidative Stress / drug effects*
  • Rats, Wistar
  • Receptors, Adrenergic, beta / metabolism*
  • Signal Transduction / drug effects
  • Superoxide Dismutase / metabolism
  • Time Factors
  • Ventricular Function, Left / drug effects

Substances

  • Antioxidants
  • Biomarkers
  • Receptors, Adrenergic, beta
  • Malondialdehyde
  • Apigenin
  • Catalase
  • Superoxide Dismutase
  • Creatine Kinase, MB Form
  • Glutathione
  • Isoproterenol