A method to analyze, sort, and retain viability of obligate anaerobic microorganisms from complex microbial communities

J Microbiol Methods. 2015 Oct:117:74-7. doi: 10.1016/j.mimet.2015.07.009. Epub 2015 Jul 14.

Abstract

A high speed flow cytometric cell sorter was modified to maintain a controlled anaerobic environment. This technology enabled coupling of the precise high-throughput analytical and cell separation capabilities of flow cytometry to the assessment of cell viability of evolved lineages of obligate anaerobic organisms from cocultures.

Keywords: Anaerobic microorganism; Controlled-environment sorting; Flow cytometry; Single cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacteria, Anaerobic / cytology
  • Bacteria, Anaerobic / isolation & purification*
  • Bacteriological Techniques / instrumentation
  • Bacteriological Techniques / methods*
  • Equipment Design
  • Flow Cytometry / instrumentation
  • Flow Cytometry / methods*
  • Microbial Viability*
  • Single-Cell Analysis