Innate and adaptive cellular phenotypes contributing to pulmonary disease in mice after respiratory syncytial virus immunization and infection

Young-Tae Lee; Ki-Hye Kim; Hye Suk Hwang; Youri Lee; Young-Man Kwon; Eun-Ju Ko; Yu-Jin Jung; Yu-Na Lee; Min-Chul Kim; Sang-Moo Kang

doi:10.1016/j.virol.2015.07.001

Innate and adaptive cellular phenotypes contributing to pulmonary disease in mice after respiratory syncytial virus immunization and infection

Virology. 2015 Nov:485:36-46. doi: 10.1016/j.virol.2015.07.001. Epub 2015 Jul 18.

Authors

Affiliations

¹ Center for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30303, USA.
² Center for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30303, USA; Department of Biology, Georgia State University, Atlanta, GA 30303, USA.
³ Center for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30303, USA; Animal and Plant Quarantine Agency, 175 Anyangro, Anyangsi, Gyeonggido 430-757, Korea.
⁴ Center for Inflammation, Immunity & Infection, Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30303, USA; Department of Biology, Georgia State University, Atlanta, GA 30303, USA. Electronic address: [email protected].

Abstract

Respiratory syncytial virus (RSV) is the major leading cause of infantile viral bronchiolitis. However, cellular phenotypes contributing to the RSV protection and vaccine-enhanced disease remain largely unknown. Upon RSV challenge, we analyzed phenotypes and cellularity in the lung of mice that were naïve, immunized with formalin inactivated RSV (FI-RSV), or re-infected with RSV. In comparison with naïve and live RSV re-infected mice, the high levels of eosinophils, neutrophils, plasmacytoid and CD11b(+) dendritic cells, and IL-4(+) CD4(+) T cells were found to be contributing to pulmonary inflammation in FI-RSV immune mice despite lung viral clearance. Alveolar macrophages appeared to play differential roles in protection and inflammation upon RSV infection of different RSV immune mice. These results suggest that multiple innate and adaptive immune components differentially contribute to RSV disease and inflammation.

Keywords: Alveolar macrophages; Clodronate liposome; Formalin-inactivated RSV; Respiratory syncytial virus (RSV); Vaccine.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Adaptive Immunity
Animals
CD4-Positive T-Lymphocytes / immunology
CD4-Positive T-Lymphocytes / pathology
CD4-Positive T-Lymphocytes / virology
Clodronic Acid / pharmacology
Dendritic Cells / immunology
Dendritic Cells / pathology
Dendritic Cells / virology
Eosinophils / immunology
Eosinophils / pathology
Eosinophils / virology
Female
Formaldehyde
Immunity, Innate
Immunization
Interleukin-4 / biosynthesis
Liposomes / pharmacology
Lung / drug effects
Lung / immunology
Lung / pathology*
Lung / virology
Macrophages, Alveolar / drug effects
Macrophages, Alveolar / immunology
Macrophages, Alveolar / pathology*
Macrophages, Alveolar / virology
Mice
Mice, Inbred BALB C
Neutrophils / immunology
Neutrophils / pathology
Neutrophils / virology
Phenotype
Respiratory Syncytial Virus Infections / immunology
Respiratory Syncytial Virus Infections / pathology*
Respiratory Syncytial Virus Infections / prevention & control*
Respiratory Syncytial Virus Infections / virology
Respiratory Syncytial Virus Vaccines / administration & dosage*
Respiratory Syncytial Viruses
Vaccines, Inactivated

Substances

Liposomes
Respiratory Syncytial Virus Vaccines
Vaccines, Inactivated
Clodronic Acid
Formaldehyde
Interleukin-4

Abstract

Publication types

MeSH terms

Substances

Grants and funding