An Improved Method With High Specificity for KIR2DL1 Functional Allele Typing

Lab Med. 2015 Summer;46(3):207-13. doi: 10.1309/LMMN1K6SBRJCGD9J.

Abstract

As new killer-cell immunoglobulin-like receptor (KIR) alleles are discovered, a challenge in KIR typing is maintaining sensitivity and specificity. A single nucleotide polymorphism assay can be used to type functional KIR2DL1 alleles. We improved recently on the earlier method by using a higher-specificity assay. The major modifications include the development of sequence-specific primers to selectively amplify the transmembrane domain of all known KIR2DL1 alleles via polymerase chain reaction with sequence-specific primers (PCR-SSP), and using the PCR products as the template for a revised KIR2DL1 functional allele-typing assay. This modified method allows high-throughput typing with high specificity.

Keywords: KIR polymorphism; KIR2DL1 allelotyping; KIRs genotyping; killer-cell immunoglobulin-like receptor (KIR); natural killer (NK) cell; single nucleotide polymorphism (SNP) assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Genotype
  • Humans
  • Polymorphism, Single Nucleotide / genetics*
  • Receptors, KIR / genetics
  • Receptors, KIR / metabolism
  • Receptors, KIR2DL1 / genetics*
  • Sensitivity and Specificity
  • Sequence Analysis, DNA / methods*

Substances

  • KIR2DL1 protein, human
  • Receptors, KIR
  • Receptors, KIR2DL1