Single cell measurement of telomerase expression and splicing using microfluidic emulsion cultures

Nucleic Acids Res. 2015 Sep 18;43(16):e104. doi: 10.1093/nar/gkv477. Epub 2015 Jul 21.

Abstract

Telomerase is a reverse transcriptase that maintains telomeres on the ends of chromosomes, allowing rapidly dividing cells to proliferate while avoiding senescence and apoptosis. Understanding telomerase gene expression and splicing at the single cell level could yield insights into the roles of telomerase during normal cell growth as well as cancer development. Here we use droplet-based single cell culture followed by single cell or colony transcript abundance analysis to investigate the relationship between cell growth and transcript abundance of the telomerase genes encoding the RNA component (hTR) and protein component (hTERT) as well as hTERT splicing. Jurkat and K562 cells were examined under normal cell culture conditions and during exposure to curcumin, a natural compound with anti-carcinogenic and telomerase activity-reducing properties. Individual cells predominantly express single hTERT splice variants, with the α+/β- variant exhibiting significant transcript abundance bimodality that is sustained through cell division. Sub-lethal curcumin exposure results in reduced bimodality of all hTERT splice variants and significant upregulation of alpha splicing, suggesting a possible role in cellular stress response. The single cell culture and transcript abundance analysis method presented here provides the tools necessary for multiparameter single cell analysis which will be critical for understanding phenotypes of heterogeneous cell populations, disease cell populations and their drug response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Curcumin / pharmacology
  • Gene Expression / drug effects
  • Humans
  • Jurkat Cells
  • K562 Cells
  • Microfluidic Analytical Techniques / methods*
  • RNA / analysis
  • RNA Splicing / drug effects*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Single-Cell Analysis*
  • Telomerase / analysis
  • Telomerase / genetics*
  • Telomerase / metabolism

Substances

  • Antineoplastic Agents
  • telomerase RNA
  • RNA
  • Telomerase
  • Curcumin