Abstract
We developed a quantum-dot-based fluorescence resonance energy transfer (QD-FRET) nanosensor to visualize the activity of matrix metalloproteinase (MT1-MMP) at cell membrane. A bended peptide with multiple motifs was engineered to position the FRET pair at a close proximity to allow energy transfer, which can be cleaved by active MT1-MMP to result in FRET changes and the exposure of cell penetrating sequence. Via FRET and penetrated QD signals, the nanosensor can profile cancer cells.
Keywords:
FRET nanosensor; MT1-MMP; activatable cell-penetrating peptide; cancer; multiplex signals; single cell.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Biosensing Techniques / methods
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Cell Line, Tumor
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Female
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Fluorescence Resonance Energy Transfer / methods*
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / metabolism*
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HeLa Cells
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Humans
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Matrix Metalloproteinase 14 / analysis
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Matrix Metalloproteinase 14 / metabolism*
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Models, Molecular
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Molecular Sequence Data
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Neoplasms / enzymology*
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Peptides / chemistry
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Peptides / metabolism*
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Quantum Dots / chemistry
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Quantum Dots / metabolism
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Single-Cell Analysis / methods*
Substances
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Fluorescent Dyes
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Peptides
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Matrix Metalloproteinase 14