Usefulness of pulsed-field gel electrophoresis profiles for the determination of Salmonella serovars

Chien-Shun Chiou; Mia Torpdahl; Ying-Shu Liao; Chun-Hsing Liao; Chi-Sen Tsao; Shiu-Yun Liang; You-Wun Wang; Jung-Che Kuo; Yen-Yi Liu

doi:10.1016/j.ijfoodmicro.2015.07.016

Usefulness of pulsed-field gel electrophoresis profiles for the determination of Salmonella serovars

Int J Food Microbiol. 2015 Dec 2:214:1-3. doi: 10.1016/j.ijfoodmicro.2015.07.016. Epub 2015 Jul 17.

Authors

Chien-Shun Chiou¹, Mia Torpdahl², Ying-Shu Liao³, Chun-Hsing Liao⁴, Chi-Sen Tsao⁵, Shiu-Yun Liang⁶, You-Wun Wang⁷, Jung-Che Kuo⁸, Yen-Yi Liu⁹

Affiliations

¹ Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: [email protected].
² Statens Serum Institut, DK-2300 Copenhagen S, Denmark. Electronic address: [email protected].
³ Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: [email protected].
⁴ Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: [email protected].
⁵ Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: [email protected].
⁶ Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: [email protected].
⁷ Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: [email protected].
⁸ Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: [email protected].
⁹ Center for Research, Diagnostics and Vaccine Development, Centers for Disease Control, Taichung 40855, Taiwan. Electronic address: [email protected].

PMID: 26208096
DOI: 10.1016/j.ijfoodmicro.2015.07.016

Abstract

We created a database consisting of a large number of Salmonella pulsed-field gel electrophoresis (PFGE) profiles covering a wide range of different serovars. This database was used for the prediction of the serovars based on the PFGE profiles for isolates from Taiwan and Denmark. The PFGE profiles proved very useful in the determination of a serovar although serovar prediction was more efficient for local isolates than those from a distant geographic area. To use a highly stringent band matching tolerance in the BioNumerics software is also important for the grouping of serovars.

Keywords: Molecular serotyping; Pulsed-field gel electrophoresis; Salmonella.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques / methods*
Bacterial Typing Techniques / standards*
Denmark
Electrophoresis, Gel, Pulsed-Field*
Salmonella / classification*
Salmonella / genetics*
Serogroup
Taiwan