Investigation of mechanisms and molecular epidemiology of linezolid nonsusceptible Enterococcus faecalis isolated from a teaching hospital in China

Bin Li; Chuan-Ling Ma; Xiao Yu; Yao Sun; Mei-Mei Li; Jian-Zhong Ye; Ya-Pei Zhang; Qing Wu; Tie-Li Zhou

doi:10.1016/j.jmii.2015.05.010

Investigation of mechanisms and molecular epidemiology of linezolid nonsusceptible Enterococcus faecalis isolated from a teaching hospital in China

J Microbiol Immunol Infect. 2016 Aug;49(4):595-9. doi: 10.1016/j.jmii.2015.05.010. Epub 2015 Jun 27.

Authors

Bin Li¹, Chuan-Ling Ma¹, Xiao Yu², Yao Sun¹, Mei-Mei Li¹, Jian-Zhong Ye¹, Ya-Pei Zhang¹, Qing Wu¹, Tie-Li Zhou³

Affiliations

¹ Department of Clinical Laboratory, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
² School of Laboratory Medicine and life Science, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
³ Department of Clinical Laboratory, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China. Electronic address: [email protected].

PMID: 26210761
DOI: 10.1016/j.jmii.2015.05.010

Abstract

The epidemiological and molecular characteristics of eight linezolid nonsusceptible Enterococcus faecalis isolated from a teaching hospital in China (January to July 2014) were investigated. The target site modifications and cfr gene associated with linezolid resistance were not found. Results of the epidemiological investigation indicated that linezolid resistance possibly occurred on several independent occasions and was often not related to linezolid administration.

Keywords: 23S rRNA; Enterococcus faecalis; Linezolid nonsusceptible; Multilocus sequence typing; Pulsed-field gel electrophoresis.

MeSH terms

Adult
Aged, 80 and over
Anti-Bacterial Agents / pharmacology*
China / epidemiology
Drug Resistance, Bacterial / genetics*
Electrophoresis, Gel, Pulsed-Field
Enterococcus faecalis / drug effects*
Enterococcus faecalis / genetics*
Enterococcus faecalis / isolation & purification
Female
Gram-Positive Bacterial Infections / drug therapy
Gram-Positive Bacterial Infections / epidemiology
Gram-Positive Bacterial Infections / microbiology*
Hospitals, Teaching
Humans
Linezolid / pharmacology*
Male
Membrane Transport Proteins / genetics*
Microbial Sensitivity Tests
Middle Aged
Molecular Epidemiology
Multilocus Sequence Typing
RNA, Ribosomal, 23S / genetics

Substances

Anti-Bacterial Agents
Membrane Transport Proteins
RNA, Ribosomal, 23S
Linezolid