In vivo comet assay of acrylonitrile, 9-aminoacridine hydrochloride monohydrate and ethanol in rats

Mutat Res Genet Toxicol Environ Mutagen. 2015 Jul:786-788:104-13. doi: 10.1016/j.mrgentox.2015.04.001. Epub 2015 Apr 8.

Abstract

As part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative international validation study of the in vivo rat alkaline comet assay, we examined the ability of acrylonitrile, 9-aminoacridine hydrochloride monohydrate (9-AA), and ethanol to induce DNA damage in the liver and glandular stomach of male rats. Acrylonitrile is a genotoxic carcinogen, 9-AA is a genotoxic non-carcinogen, and ethanol is a non-genotoxic carcinogen. Positive results were obtained in the liver cells of male rats treated with known genotoxic compounds, acrylonitrile and 9-AA.

Keywords: 9-Aminoacridine hydrochloride monohydrate; Acrylonitrile; Ethanol; In vivo rat comet assay; JaCVAM validation study.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Acrylonitrile / toxicity*
  • Aminacrine / toxicity*
  • Animals
  • Carcinogens / toxicity
  • Comet Assay / methods*
  • DNA Damage / drug effects
  • Dose-Response Relationship, Drug
  • Ethanol / toxicity*
  • Liver / drug effects
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Stomach / drug effects

Substances

  • Carcinogens
  • Ethanol
  • Aminacrine
  • Acrylonitrile