A sensitive method for assay of a novel tricyclic compound using coulometric electrochemical detection

J Pharm Sci. 1989 Nov;78(11):971-4. doi: 10.1002/jps.2600781119.

Abstract

A reversed-phase high-performance liquid chromatographic method using coulometric electrochemical detection in the oxidative mode has been developed for the analysis of 3-(9-chloro-5,6-dihydro-11-H-pyrrolo[2,1-b][3]benzazepine-11-ylidene- N,N-dimethyl-1-propanamine(E)-Z-butenedioate hydrogen maleate (1) in plasma of patients dosed with 2-8 mg/kg/d of the drug. Concentrations as little as 0.1 ng/mL of 1 in plasma can be estimated with a mean coefficient of variation of 7.4 +/- 1.08%. The utility of the procedure was demonstrated by the analysis of 500 patient samples from a rising multiple-dose study.

MeSH terms

  • Antidepressive Agents, Tricyclic / analysis*
  • Antidepressive Agents, Tricyclic / blood
  • Benzazepines / analysis*
  • Benzazepines / blood
  • Chromatography, High Pressure Liquid
  • Electrochemistry
  • Humans

Substances

  • Antidepressive Agents, Tricyclic
  • Benzazepines
  • 3-(9-chloro-5,6-dihydro-11-H-pyrrolo(2,1-b))(3)benzazepine-11-ylidine-N,N-dimethyl-1-propanaminebutenedioate