In Situ Biosynthesis of Fluorescent Platinum Nanoclusters: Toward Self-Bioimaging-Guided Cancer Theranostics

ACS Appl Mater Interfaces. 2015 Aug 19;7(32):18163-9. doi: 10.1021/acsami.5b05805. Epub 2015 Aug 7.

Abstract

Among the noble-metal clusters, very few reports about platinum clusters were used as bioimaging probes of tumors except as a reducing catalyst. It is first established herein that the biocompatible platinum nanoclusters are spontaneously biosynthesized by cancerous cells (i.e., HepG2 (human hepatocarcinoma), A549 (lung cancer), and others) rather than noncancerous cells (i.e., L02 (human embryo liver cells)) when incubated with micromolar chloroplatinic acid solutions. These in situ biosynthesized platinum nanoclusters could be readily realized in a biological environment and emit a bright fluorescence at 460 nm, which could be further utilized to facilitate an excellent cancer-cell-killing efficiency when combined with porphyrin derivatives for photothermal treatment. This raises the possibility of providing a promising and precise bioimaging strategy for specific fluorescent self-biomarking of tumor locations and realizing fluorescence imaging-guided photothermal therapy of tumors.

Keywords: bioimaging; cancer; photothermal treatment; platinum nanoclusters; theranostics.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Fluorescent Dyes / chemistry*
  • HCT116 Cells
  • HeLa Cells
  • Hep G2 Cells
  • Humans
  • Infrared Rays
  • Metal Nanoparticles / chemistry*
  • Metal Nanoparticles / therapeutic use
  • Mice
  • Mice, Nude
  • Microscopy, Confocal
  • Neoplasms / pathology
  • Neoplasms / therapy
  • Phototherapy
  • Platinum / chemistry*
  • Platinum Compounds / chemistry
  • Porphyrins / chemistry
  • Reactive Oxygen Species / metabolism
  • Theranostic Nanomedicine
  • Transplantation, Heterologous

Substances

  • Fluorescent Dyes
  • Platinum Compounds
  • Porphyrins
  • Reactive Oxygen Species
  • chloroplatinic acid
  • Platinum