A source for microhydrated biomolecules

Rev Sci Instrum. 2015 Jul;86(7):073103. doi: 10.1063/1.4923247.

Abstract

We describe the construction of an apparatus for the production of a molecular jet of microhydrated biomolecules. Our design uses a water reservoir producing water vapour, which then passes through a separate reservoir containing a vapour of a sublimated biomolecule. The mixture coexpands into a molecular beam apparatus through a conical nozzle. Mass spectra showing water-adenin and water-uracil complexes are shown as typical examples. Suitable expansion conditions are reached without the use of an inert carrier gas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine* / chemistry
  • Equipment Design
  • Gases / chemistry
  • Microtechnology / instrumentation*
  • Protons
  • Spectrum Analysis
  • Sublimation, Chemical*
  • Synchrotrons
  • Uracil* / chemistry
  • Vacuum
  • Water* / chemistry

Substances

  • Gases
  • Protons
  • Water
  • Uracil
  • Adenine