Standardizing metadata and taxonomic identification in metabarcoding studies

Gigascience. 2015 Jul 31:4:34. doi: 10.1186/s13742-015-0074-5. eCollection 2015.

Abstract

High-throughput sequencing-based metabarcoding studies produce vast amounts of ecological data, but a lack of consensus on standardization of metadata and how to refer to the species recovered severely hampers reanalysis and comparisons among studies. Here we propose an automated workflow covering data submission, compression, storage and public access to allow easy data retrieval and inter-study communication. Such standardized and readily accessible datasets facilitate data management, taxonomic comparisons and compilation of global metastudies.

Keywords: Data storage; Digital object identifiers (DOI); Environmental metadata; High-throughput sequencing (HTS); Interactive database; Internal transcribed spacer (ITS); Next-generation sequencing; Species hypotheses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodiversity
  • DNA Barcoding, Taxonomic*
  • High-Throughput Nucleotide Sequencing
  • Sequence Alignment