Abstract
We report on transiently responsive protein-polymer conjugates that temporarily change their protein conformation from the soluble to the particle-like state. 'Grafting-from' RAFT polymerization of a dioxolane-containing acrylamide with a protein macroCTA is used to design polymer-protein conjugates that self-assemble into nanoparticles at physiological temperature and pH. Acid triggered hydrolysis of the dioxolane units into diol moeities rendered the conjugates fully water soluble irrespective of temperature.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acrylamides / administration & dosage
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Acrylamides / chemistry*
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Animals
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Cell Line
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Dendritic Cells / metabolism
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Dioxolanes / chemistry
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Hydrogen-Ion Concentration
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Hydrolysis
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Immunologic Factors / administration & dosage
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Immunologic Factors / chemistry*
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Mice
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Nanoparticles / administration & dosage
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Nanoparticles / chemistry*
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Polymerization
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Polymers / administration & dosage
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Polymers / chemistry*
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Protein Conformation
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Quinolines / administration & dosage
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Quinolines / chemistry*
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Serum Albumin, Bovine / administration & dosage
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Serum Albumin, Bovine / chemistry*
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Solubility
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Temperature
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Thiazoles / administration & dosage
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Thiazoles / chemistry*
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Water / chemistry
Substances
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Acrylamides
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CL 075
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Dioxolanes
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Immunologic Factors
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Polymers
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Quinolines
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Thiazoles
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poly-N-((2,2-dimethyl-1,3-dioxolane)methyl)acrylamide
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Water
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Serum Albumin, Bovine
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formal glycol