Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Biomaterials. 2015 Nov:69:45-55. doi: 10.1016/j.biomaterials.2015.08.009. Epub 2015 Aug 5.

Abstract

Extensive urethral defects have a serious impact on quality of life, and treatment is challenging. A shortage of material for reconstruction is a key limitation. Improving the properties of biomaterials and making them suitable for urethral reconstruction will be helpful. Previously, we constructed a fusion protein, collagen-binding VEGF (CBD-VEGF), which can bind to collagen scaffold, stimulate cell proliferation, and promote angiogenesis and tissue regeneration. We proposed that CBD-VEGF could improve the performance of collagen in reconstruction of extensive urethral defects. Our results showed that collagen scaffolds modified with CBD-VEGF could promote urethral tissue regeneration and improve the function of the neo-urethra in a beagle extensive urethral defect model. Thus, modifying biomaterials with bioactive factors provides an alternative strategy for the production of suitable biomaterials for urethral reconstruction.

Keywords: Collagen; Collagen-binding domain; Urethral regeneration; Vascular endothelial growth factors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Collagen / chemistry*
  • Dogs
  • Male
  • Plastic Surgery Procedures
  • Quality of Life
  • Regeneration*
  • Tissue Scaffolds / chemistry*
  • Urethra / drug effects
  • Urethra / injuries*
  • Urethra / physiology*
  • Urethra / surgery
  • Vascular Endothelial Growth Factor A / administration & dosage
  • Vascular Endothelial Growth Factor A / therapeutic use*

Substances

  • Vascular Endothelial Growth Factor A
  • Collagen