Phenylalanine interaction with lipid monolayers at different pHs

A C Cutró; A Hollmann; J Cejas; P Maturana; E A Disalvo; M A Frías

doi:10.1016/j.colsurfb.2015.07.059

Phenylalanine interaction with lipid monolayers at different pHs

Colloids Surf B Biointerfaces. 2015 Nov 1:135:504-509. doi: 10.1016/j.colsurfb.2015.07.059. Epub 2015 Jul 26.

Authors

A C Cutró¹, A Hollmann¹, J Cejas¹, P Maturana¹, E A Disalvo², M A Frías¹

Affiliations

¹ Laboratorio de Biointerfases y Sistemas Biomiméticos, Laboratorios Centrales, CITSE-UNSE, Santiago del Estero, Argentina.
² Laboratorio de Biointerfases y Sistemas Biomiméticos, Laboratorios Centrales, CITSE-UNSE, Santiago del Estero, Argentina. Electronic address: [email protected].

PMID: 26283499
DOI: 10.1016/j.colsurfb.2015.07.059

Abstract

The influence of Phe on the surface pressure of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) monolayers at the air-water interface was studied at different initial surface pressures (26 and 40 mN/m) and two pHs (5.0 and 7.3) at constant temperature (20 °C). Changes produced by the aminoacid added to the subphase on the surface pressure and on the dipole potential of lipid monolayers were measured at a fixed area. Compressibility properties of the monolayers at different pHs were studied by (π-A) isotherms. The results suggest that Phe intercalates into a DPPC film at the air-water interface at pH 5 and forms a different arrangement at pH 7.3. The possible relevance of these results of the effect of Phe in physiological conditions is discussed.

Keywords: DPPC; Dipole potential-pH; Lipid monolayers; Phenylalanine; Surface pressure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

1,2-Dipalmitoylphosphatidylcholine / chemistry
Algorithms
Hydrogen-Ion Concentration
Lipids / chemistry*
Membranes, Artificial
Phenylalanine / chemistry*
Surface Properties
Temperature
Thermodynamics

Substances

Lipids
Membranes, Artificial
1,2-Dipalmitoylphosphatidylcholine
Phenylalanine