Abstract
The spatial positioning of 3D tumor-mimetic microenvironments, containing multiple cell types, is controlled with a simple concentric flow device in a single step. A range of geometric architectures are demonstrated, and the migration of segregated tumor cells and macrophages is explored using drugs that inhibit heterotypic interactions.
Keywords:
microfluidics; patterned hydrogels; tissue engineering; tumor microenvironments.
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Adenocarcinoma / drug therapy
-
Adenocarcinoma / physiopathology
-
Alginates / chemical synthesis
-
Alginates / chemistry
-
Animals
-
Biomimetic Materials / chemical synthesis
-
Biomimetic Materials / chemistry
-
Breast Neoplasms / drug therapy
-
Breast Neoplasms / physiopathology
-
Cell Line, Tumor
-
Cell Movement / drug effects
-
Cell Movement / physiology
-
Cell Survival / drug effects
-
Cell Survival / physiology
-
Coculture Techniques
-
Equipment Design
-
Glucuronic Acid / chemical synthesis
-
Glucuronic Acid / chemistry
-
Hexuronic Acids / chemical synthesis
-
Hexuronic Acids / chemistry
-
Humans
-
Hydrogels / chemical synthesis
-
Hydrogels / chemistry
-
Lab-On-A-Chip Devices
-
Macrophages / drug effects
-
Macrophages / physiology
-
Mice
-
Microfluidics / instrumentation
-
Microfluidics / methods*
-
Tissue Scaffolds*
-
Tumor Microenvironment / drug effects*
-
Tumor Microenvironment / physiology*
Substances
-
Alginates
-
Hexuronic Acids
-
Hydrogels
-
Glucuronic Acid