Mycobacterium tuberculosis pncA Polymorphisms That Do Not Confer Pyrazinamide Resistance at a Breakpoint Concentration of 100 Micrograms per Milliliter in MGIT

J Clin Microbiol. 2015 Nov;53(11):3633-5. doi: 10.1128/JCM.01001-15. Epub 2015 Aug 19.

Abstract

Sequencing of the Mycobacterium tuberculosis pncA gene allows for pyrazinamide susceptibility testing. We summarize data on pncA polymorphisms that do not confer resistance at a susceptibility breakpoint of 100 μg/ml pyrazinamide in MGIT within a cohort of isolates from South Africa and the U.S. Centers for Disease Control and Prevention.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amidohydrolases / genetics*
  • Antitubercular Agents / pharmacology*
  • Base Sequence
  • DNA, Bacterial / genetics
  • Drug Resistance, Bacterial / genetics
  • Humans
  • Microbial Sensitivity Tests
  • Mycobacterium tuberculosis / drug effects*
  • Mycobacterium tuberculosis / genetics*
  • Mycobacterium tuberculosis / isolation & purification
  • Polymorphism, Single Nucleotide / genetics
  • Pyrazinamide / pharmacology*
  • Sequence Analysis, DNA
  • South Africa
  • United States

Substances

  • Antitubercular Agents
  • DNA, Bacterial
  • Pyrazinamide
  • Amidohydrolases
  • PncA protein, Mycobacterium tuberculosis
  • pyrazinamide deamidase